| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.123885 |
| Chromosome: | chromosome 5 |
| Location: | 1962419 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241638 | (1 of 1) IPR000253//IPR002562//IPR012337 - Forkhead-associated (FHA) domain // 3'-5' exonuclease domain // Ribonuclease H-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACGTTGTCGCCGCATACCTATACGTCG |
| Internal bar code: | GCATATACAAGGTTGCCCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 590 |
| LEAP-Seq percent confirming: | 98.8077 |
| LEAP-Seq n confirming: | 663 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCGCAGGTAGAGCACTTT |
| Suggested primer 2: | CGTAACACCCCTCATCTCGT |