| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.123890 |
| Chromosome: | chromosome 17 |
| Location: | 1636977 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708050 | (1 of 1) PTHR23257:SF297 - PROTO-ONCOGENE SERINE/THREONINE-PROTEIN KINASE MOS | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGAAAGCGAGGGGGGAGCGGAGCAGGT |
| Internal bar code: | TGGACCGAGTACCGTAGCCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1010 |
| LEAP-Seq percent confirming: | 67.8571 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGACTGGCCGTACGAAT |
| Suggested primer 2: | GCCCAAACTAAATCGAACCA |