| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.123907 |
| Chromosome: | chromosome 8 |
| Location: | 1552062 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g365000 | CSE1 | Protein of unknown function; (1 of 3) PF07887 - Calmodulin binding protein-like (Calmodulin_bind) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACAGCGGAGCCGTTGGGCGGATGCCAC |
| Internal bar code: | TATGGCCAGAAGAATCGGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 612 |
| LEAP-Seq percent confirming: | 99.8278 |
| LEAP-Seq n confirming: | 11013 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGATGAGTGCGAGCCAC |
| Suggested primer 2: | GCAAAGGAGCTCAACCTGAC |