| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.123908 |
| Chromosome: | chromosome 17 |
| Location: | 1232167 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705150 | (1 of 1) PTHR31399:SF0 - DNA-DIRECTED PRIMASE/POLYMERASE PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCAGCGCCGCCATGGGAGGCTGCGGAGT |
| Internal bar code: | CGGAGATGCGTCCCGCGTGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 911 |
| LEAP-Seq percent confirming: | 99.1736 |
| LEAP-Seq n confirming: | 360 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGATGTTGCACAAGGG |
| Suggested primer 2: | TCTGATCACAGGCTTGTTGC |