| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.123909 |
| Chromosome: | chromosome 6 |
| Location: | 2004606 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264150 | MAW11 | Membrane-associated hydroxyproline-rich glycoprotein 11; (1 of 1) 2.1.1.53 - Putrescine N-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGTGCTGTGCGCGCGTGTCAGCTACCCC |
| Internal bar code: | TCTAGGTGCCCGGACGAAAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 67 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGAAGGGGCTGCTAC |
| Suggested primer 2: | GTCCCTGACGCTTACACCAT |