| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.123943 |
| Chromosome: | scaffold 18 |
| Location: | 30435 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g748347 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCAGAAGCGAGCTGCGGGCTGGCCCCT |
| Internal bar code: | GGGTGGGGGAGGGCAGACAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 957 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGCCGTTGTATATGTGC |
| Suggested primer 2: | AACAAAAGCTGCTAGGGCAA |