| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.123970 |
| Chromosome: | chromosome 12 |
| Location: | 8614233 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547100 | CGL2,SMM43 | (1 of 2) PTHR10108//PTHR10108:SF788 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED; putative methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTAGTTTGCCCCTCCCCCCGCACCTTG |
| Internal bar code: | GGTACAGCAGAGAGAACGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 899 |
| LEAP-Seq percent confirming: | 98.6217 |
| LEAP-Seq n confirming: | 644 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGCCTAGCCAGACAGAA |
| Suggested primer 2: | CTCATGCTGATTACGGCTGA |