Insertion junction: LMJ.RY0402.123996_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.123996
Chromosome:chromosome_9
Location:3258918
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g387912 FAP139 Flagellar Associated Protein, serine endopeptidase sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):AGGTACCGTGGTGCAGTAGCGTGTGGGGAT
Internal bar code:AGTCTCTGTGGGCTCGGCGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:338
LEAP-Seq percent confirming:99.8688
LEAP-Seq n confirming:3044
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGCCTTTCAAGCTCTACG
Suggested primer 2:ATTGACCAGGTTGAAGTCCG