Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.123996 |
Chromosome: | chromosome_9 |
Location: | 3258918 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g387912 | FAP139 | Flagellar Associated Protein, serine endopeptidase | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGGTACCGTGGTGCAGTAGCGTGTGGGGAT |
Internal bar code: | AGTCTCTGTGGGCTCGGCGTGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 338 |
LEAP-Seq percent confirming: | 99.8688 |
LEAP-Seq n confirming: | 3044 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTGCCTTTCAAGCTCTACG |
Suggested primer 2: | ATTGACCAGGTTGAAGTCCG |