Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.123996 |
Chromosome: | chromosome_9 |
Location: | 3258922 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g387912 | FAP139 | Flagellar Associated Protein, serine endopeptidase | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACCAACCAAAGCCCGCGCCCTCACAGCCCC |
Internal bar code: | CACAAGGTGTAGAGCTGGCTCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 852 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 17 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTGCCTTTCAAGCTCTACG |
Suggested primer 2: | ATTGACCAGGTTGAAGTCCG |