| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124009 |
| Chromosome: | chromosome 8 |
| Location: | 1330856 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g363874 | (1 of 3) 2.7.9.4 - Alpha-glucan, water dikinase / Starch-related R1 protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGAAGGGCTCGGGAACGGCTTTCCCCCT |
| Internal bar code: | TCTTCGCGCCGTGCACGGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 240 |
| LEAP-Seq percent confirming: | 98.9655 |
| LEAP-Seq n confirming: | 287 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGACATCACCGCCTACTG |
| Suggested primer 2: | TTTAACACAAGGAAACCCGC |