| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124011 |
| Chromosome: | chromosome 13 |
| Location: | 5142765 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607650 | POC15 | Proteome of centriole protein 15; (1 of 1) PTHR10891//PTHR10891:SF5 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTGGTGGGTCCTATCAAGAGACATTGC |
| Internal bar code: | TAACTGGAGATTTCATGAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 91.8147 |
| LEAP-Seq n confirming: | 1447 |
| LEAP-Seq n nonconfirming: | 129 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAATCTTGCTTGCACCTG |
| Suggested primer 2: | GACTTGGATTGATGGCAGGT |