Insertion junction: LMJ.RY0402.124026_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.124026
Chromosome:scaffold 24
Location:64559
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre24.g755597 Chlorophyll a /Pheophorbide a oxygenase-related protein; (1 of 2) 1.14.13.122 - Chlorophyllide-a oxygenase / Cholorophyll-b synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCCCACAGCACCACAACCATACACGTCA
Internal bar code:GACAGGGCGGACGTTCTAGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:170
LEAP-Seq percent confirming:99.4475
LEAP-Seq n confirming:540
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTTGAAGCATGCAAATCC
Suggested primer 2:CACTCCTACCCTCCTCCCTC