| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124067 |
| Chromosome: | chromosome 9 |
| Location: | 353405 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g405100 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGGTGGCAGTGGTCGCACTCGGGTTCC |
| Internal bar code: | TCTGCCGCCTGAGAACACGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 782 |
| LEAP-Seq percent confirming: | 99.8901 |
| LEAP-Seq n confirming: | 3634 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCCCTGGCAAGACACTC |
| Suggested primer 2: | TGCGCAGTAGAGGGTGTATG |