| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.124085 |
| Chromosome: | chromosome 14 |
| Location: | 1540652 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618600 | MRP7 | ABC transporter, multidrug resistance associated protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGATCCAGCAACGCCCCCCAGTCCTGC |
| Internal bar code: | GATGGGCCTATTCTTGCCCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 663 |
| LEAP-Seq percent confirming: | 98.2759 |
| LEAP-Seq n confirming: | 171 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGAGCTTACACCGGAAAG |
| Suggested primer 2: | AGAATGAGACCCACAGGTCG |