Insertion junction: LMJ.RY0402.124089_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.124089
Chromosome:chromosome 3
Location:1971044
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g155700 (1 of 2) PTHR22840//PTHR22840:SF12 - FAMILY NOT NAMED // WD REPEAT-CONTAINING PROTEIN 36 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTGCTGCTAGCGCCGATAAGCTCGAGAC
Internal bar code:GACGATAACGTGCCCGAAGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:794
LEAP-Seq percent confirming:99.4138
LEAP-Seq n confirming:848
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ATCACTACTGCCGCCATCTC
Suggested primer 2:GCAATCCTCGTTTGTTTGGT