| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124145 |
| Chromosome: | chromosome 9 |
| Location: | 3373552 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388763 | (1 of 1) PTHR14226//PTHR14226:SF32 - NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATTGCCTATAGCCCGACCGCTGATAGC |
| Internal bar code: | ATAGACTGCGTTACTGCTCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 900 |
| LEAP-Seq percent confirming: | 98.9599 |
| LEAP-Seq n confirming: | 3330 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATTACGTGGTTGATCCCG |
| Suggested primer 2: | GTTGAGAAACCCAAGCGAAG |