| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124211 |
| Chromosome: | chromosome 3 |
| Location: | 4082804 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172800 | (1 of 1) K18753 - butyrate response factor 1 (ZFP36L) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCTCCCCTGCGGCCCTGCATGTGCCGG |
| Internal bar code: | CGCACGAGTTATTGGTTCGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 780 |
| LEAP-Seq percent confirming: | 99.8681 |
| LEAP-Seq n confirming: | 4542 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGGGTAAAAAGTGCGTG |
| Suggested primer 2: | CCGTTCAACAACATGAGTGG |