| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124241 |
| Chromosome: | chromosome 7 |
| Location: | 2203975 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327150 | (1 of 2) IPR001810//IPR003347 - F-box domain // JmjC domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGAAAAAGCTCAAATACCGCATTGAAG |
| Internal bar code: | AATCATTCTGAAGGGGTCGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 98.4109 |
| LEAP-Seq n confirming: | 5140 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTATAGGGCTGGAGAAGG |
| Suggested primer 2: | CAAACACGGGCAATTAAACC |