| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.124245 |
| Chromosome: | chromosome 16 |
| Location: | 5877327 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677450 | (1 of 2) 5.1.3.15 - Glucose-6-phosphate 1-epimerase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGAAGGGCCTGGGGAGCGGGCATTTGTT |
| Internal bar code: | CCGTCGGCCTCCGTTCGGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 416 |
| LEAP-Seq percent confirming: | 97.427 |
| LEAP-Seq n confirming: | 1401 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCATGGTCAGGTGAGGGT |
| Suggested primer 2: | ACACTCTGAAGATGTGGCCC |