Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.124246 |
Chromosome: | chromosome_8 |
Location: | 3412853 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g374700 | FAP244 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGATGACACCACGCTCGCCTACGCCAGTG |
Internal bar code: | TCCCAGATGCGGCGGGGGTTAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 811 |
LEAP-Seq percent confirming: | 99.7692 |
LEAP-Seq n confirming: | 2161 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCATGCTGCTTTCTAGTCC |
Suggested primer 2: | CACACGTCCATCTGGTTGTC |