| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.124249 |
| Chromosome: | chromosome 2 |
| Location: | 4731748 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102100 | SMM5 | (1 of 2) PF13679 - Methyltransferase domain (Methyltransf_32); S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAAACTCTCAGCGGGCGTCGGAGCCTCA |
| Internal bar code: | CCGTTCGCGTTTGTTACTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 413 |
| LEAP-Seq percent confirming: | 99.8144 |
| LEAP-Seq n confirming: | 3765 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACGGTGTATGATGCCCT |
| Suggested primer 2: | CGTACCCCTCCTGTCCTACA |