| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.124260 |
| Chromosome: | chromosome 11 |
| Location: | 1899909 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468100 | PDE30 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCCAACTCGACGCTGTACGACGGAATG |
| Internal bar code: | TTGGGTTGTTCTGCGTCGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 304 |
| LEAP-Seq percent confirming: | 99.6124 |
| LEAP-Seq n confirming: | 7709 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCGTTGGTCTGAGTGTG |
| Suggested primer 2: | CAAGTTCGGTAACACTGCGA |