| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.124271 |
| Chromosome: | chromosome 17 |
| Location: | 1158557 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g704400 | (1 of 4) PTHR12298//PTHR12298:SF7 - PCDC2 PROGRAMMED CELL DEATH PROTEIN 2 -RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGCACGTTGGAGTTTTGGGCGTCCGTG |
| Internal bar code: | GCGAATACCTCTGCTCGTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 900 |
| LEAP-Seq percent confirming: | 99.7314 |
| LEAP-Seq n confirming: | 3342 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTCCCTACTTCCCCGAAA |
| Suggested primer 2: | CGGTTCCTTACCAACCTTGA |