| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124285 |
| Chromosome: | chromosome_16 |
| Location: | 1984948 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g656650 | PGP8 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AACTACGCTTCTCGCAACGCCCTGTACAAC |
| Internal bar code: | CACCTGGCGCCTTTTGGGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 841 |
| LEAP-Seq percent confirming: | 77.8579 |
| LEAP-Seq n confirming: | 2268 |
| LEAP-Seq n nonconfirming: | 645 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTACGTCGTGTTGATGG |
| Suggested primer 2: | ATCACCTACACGCCTTCCAC |