Insertion junction: LMJ.RY0402.124318_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.124318
Chromosome:chromosome 16
Location:2938546
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g664100 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTAAACTGTGTCCTCGCACCGACACGCAG
Internal bar code:GCTGACAGGTCCCCTGATGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1002
LEAP-Seq percent confirming:99.6433
LEAP-Seq n confirming:3632
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:ACTACTGGTGGCGTTTGTCC
Suggested primer 2:TAAGACATGCGTCAAGGCAG