| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124337 |
| Chromosome: | chromosome 3 |
| Location: | 9019113 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g210065 | (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTAGCAAGCCGCAATCCCTGGCGTCAAC |
| Internal bar code: | GGAGGCGGGCATACAGGGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 99.8729 |
| LEAP-Seq n confirming: | 4715 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGCACATGCAAAACATAA |
| Suggested primer 2: | GCGTGTGTGTGTGTGTGTGT |