| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.124342 |
| Chromosome: | chromosome 2 |
| Location: | 1622823 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085200 | (1 of 12) PF01585 - G-patch domain (G-patch) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTAAGCGCTAGGGTAGCCCACTCGGCGCC |
| Internal bar code: | ACATGGATTGCGACGCTGACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 379 |
| LEAP-Seq percent confirming: | 94.4314 |
| LEAP-Seq n confirming: | 1204 |
| LEAP-Seq n nonconfirming: | 71 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTTGGGGTGAGCTTTGTA |
| Suggested primer 2: | CGACTGTGACTGCTGTTGCT |