Insertion junction: LMJ.RY0402.124343_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.124343
Chromosome:chromosome 15
Location:1125842
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre15.g639504 (1 of 3) PF13637//PF13920 - Ankyrin repeats (many copies) (Ank_4) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCGAAGGTGACTACCGAAGGTGACTACC
Internal bar code:CTCGTCGTGCAGGGGCTCAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1203
LEAP-Seq percent confirming:99.8974
LEAP-Seq n confirming:1947
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGACTTGCTGCCGTCTCTAC
Suggested primer 2:AGACTAGGTGGAGCAGGGGT