Insertion junction: LMJ.RY0402.124365_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.124365
Chromosome:chromosome 5
Location:847944
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g247850 (1 of 3) 3.6.1.22//3.6.1.55 - NAD(+) diphosphatase / NADP pyrophosphatase // 8-oxo-dGTP diphosphatase / 8-oxo-dGTPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTACTCGTGGGGCTGGAGGGCGAGGACG
Internal bar code:AAGAGATGGCGGTGTACGCTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:828
LEAP-Seq percent confirming:90.1747
LEAP-Seq n confirming:11977
LEAP-Seq n nonconfirming:1305
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:GTGAAGCGGTTGGAAGAGAG
Suggested primer 2:TGTGTTTTGATCGCGTGTTT