Insertion junction: LMJ.RY0402.124374_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.124374
Chromosome:chromosome 7
Location:3954374
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g339500 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAGATGGGCGACAGCGAGCCGGAGCCGCT
Internal bar code:GTAAGCGTTTGATGACGCAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:241
LEAP-Seq percent confirming:99.4891
LEAP-Seq n confirming:2921
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GATTTTGATTGCGCCACTTT
Suggested primer 2:GAATACGCTTAGCCCTTCCC