| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.124376 |
| Chromosome: | chromosome 1 |
| Location: | 3970853 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025808 | OPR122 | OctotricoPeptide Repeat protein 122 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTATTTACAACCCCCACACCCGCCCAT |
| Internal bar code: | CATGCGGCTGGAGCTAAAAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 96 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGGTGGTCATTTGATGTG |
| Suggested primer 2: | GCTGGTAATGTGCTAGGCAG |