| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124386 |
| Chromosome: | chromosome_16 |
| Location: | 3873969 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g690100 | FAP213 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AATTCTACGTATTCTCTTAAGACGTGGTCA |
| Internal bar code: | AACGAGCCGGGCTTTCGAATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 696 |
| LEAP-Seq percent confirming: | 99.4 |
| LEAP-Seq n confirming: | 994 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGATCTCGGCAAGTGAAG |
| Suggested primer 2: | AGATTCTGCTGCTTCTTGGC |