| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.124414 |
| Chromosome: | chromosome 7 |
| Location: | 4970314 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346750 | PUS14 | (1 of 2) 5.4.99.28//5.4.99.29 - tRNA pseudouridine(32) synthase / Pseudouridine synthase RluA // 23S rRNA pseudouridine(746) synthase / 23S RNA Psi(746) synthase; RNA pseudouridine synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGTAGTGGCAGTGTTTGTGGGAGGCAAG |
| Internal bar code: | GCGTTGGCTCATCAGAGGTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 255 |
| LEAP-Seq percent confirming: | 72.2354 |
| LEAP-Seq n confirming: | 1215 |
| LEAP-Seq n nonconfirming: | 467 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACGTGTGTGTGTGTGTGT |
| Suggested primer 2: | GCCAGTGACAGTGAAGTGGA |