| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124414 |
| Chromosome: | chromosome 7 |
| Location: | 4970315 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346750 | PUS14 | (1 of 2) 5.4.99.28//5.4.99.29 - tRNA pseudouridine(32) synthase / Pseudouridine synthase RluA // 23S rRNA pseudouridine(746) synthase / 23S RNA Psi(746) synthase; RNA pseudouridine synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCCAGCACCACCGCCTCTGCCGCACCCA |
| Internal bar code: | CTATACTGCTGGCAGGGCAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 489 |
| LEAP-Seq percent confirming: | 10.0995 |
| LEAP-Seq n confirming: | 203 |
| LEAP-Seq n nonconfirming: | 1807 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACGTGTGTGTGTGTGTGT |
| Suggested primer 2: | GCCAGTGACAGTGAAGTGGA |