| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.124438 |
| Chromosome: | chromosome 7 |
| Location: | 4562339 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g343933 | EBM6 | (1 of 4) K19355 - mannan endo-1,4-beta-mannosidase (MAN); Mannan endo-1%252C4-beta-mannosidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCGCCTTCGGTGCGTGCGCTTTCTCGC |
| Internal bar code: | TTTTACCCTGCACGAAAGTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 220 |
| LEAP-Seq percent confirming: | 99.7265 |
| LEAP-Seq n confirming: | 4011 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGATACAGAGTGGGCA |
| Suggested primer 2: | TGCTTCTGCTGCTTTTCTCA |