| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124477 |
| Chromosome: | chromosome 16 |
| Location: | 5992430 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676600 | (1 of 4) IPR029041 - FAD-linked oxidoreductase-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCGCGACCAGTTGTGCGGGGCCGGGCG |
| Internal bar code: | CCCGCACAGAGCGCCTGAGTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 538 |
| LEAP-Seq percent confirming: | 85.0279 |
| LEAP-Seq n confirming: | 1982 |
| LEAP-Seq n nonconfirming: | 349 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGAGTGTAACTGCCCGCT |
| Suggested primer 2: | GCATCCAAGAAGGACAGAGC |