| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.124485 |
| Chromosome: | chromosome 2 |
| Location: | 6609275 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g117350 | MOT11,TTL2 | (1 of 1) K16599 - tubulin polyglutamylase TTLL1 [EC:6.-.-.-] (TTLL1); Tubulin tyrosine ligase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCGCACGCGGTACCCAGCACTTGACAG |
| Internal bar code: | TTTTCTCTAGTCTCACCTCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 428 |
| LEAP-Seq percent confirming: | 99.5698 |
| LEAP-Seq n confirming: | 2083 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTTCAACAGCAGAGGAG |
| Suggested primer 2: | CCCCTTGTGGTTTTCTCAAA |