| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.124486 |
| Chromosome: | chromosome 10 |
| Location: | 869141 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423850 | SRR26 | Scavenger receptor cysteine rich (SRCR) protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAAGGACGCGCGTCAGACGAAACCAGTC |
| Internal bar code: | ATGCATAAGTGGCGTGACGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 748 |
| LEAP-Seq percent confirming: | 99.8402 |
| LEAP-Seq n confirming: | 1874 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATAAACCAGCTTCGTGGT |
| Suggested primer 2: | ATTGCTGACACAGAGCGTTG |