Insertion junction: LMJ.RY0402.124489_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.124489
Chromosome:chromosome 16
Location:687296
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g691450 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGAACACACCCACACACACCCACACAAC
Internal bar code:CAGTCATAGGGACCATAGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:934
LEAP-Seq percent confirming:99.7354
LEAP-Seq n confirming:22619
LEAP-Seq n nonconfirming:60
LEAP-Seq n unique pos:85

Suggested primers for confirmation by PCR

Suggested primer 1:GGACGCAAGTACAGGTCCAT
Suggested primer 2:GCGTTCTTCAACCTACTCGC