| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.124512 |
| Chromosome: | chromosome 15 |
| Location: | 868890 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g639503 | GEX173 | (1 of 7) PTHR23257//PTHR23257:SF474 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACGTGTTCGAAGTGCCGACGCCCAGTAG |
| Internal bar code: | GATTAGAGTCCGGTTCAATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 561 |
| LEAP-Seq percent confirming: | 95.3338 |
| LEAP-Seq n confirming: | 12524 |
| LEAP-Seq n nonconfirming: | 613 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGAGAGCGAGCGAGTAG |
| Suggested primer 2: | CCACTCCCACTCACACACAC |