| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.124521 |
| Chromosome: | chromosome 12 |
| Location: | 6354058 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537450 | COX13 | (1 of 1) IPR001349 - Cytochrome c oxidase, subunit VIa; Mitochondrial cytochrome c oxidase 12 kDa subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCATATTCTTACATGCGGAGATGCGCAG |
| Internal bar code: | AGGGGATGCTAGATCACTAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 99.7096 |
| LEAP-Seq n confirming: | 5838 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGCGGCTGACTGACACAG |
| Suggested primer 2: | ACGCGCGCGACTATATAACT |