| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.124547 |
| Chromosome: | chromosome_1 |
| Location: | 450752 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g002550 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCCATAAAACCACACAGGGCTATCTATGT |
| Internal bar code: | TACTGTATCTTATTGAATTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 98.9064 |
| LEAP-Seq n confirming: | 2261 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACTGGCTGACTTGACCA |
| Suggested primer 2: | ACCCACCCACTCACTCACTC |