| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124547 |
| Chromosome: | chromosome 1 |
| Location: | 450754 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002550 | FAP335 | (1 of 1) 2.7.11.1//2.7.11.12 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // cGMP-dependent protein kinase / PKG II; Kinase-like Flagellar Associated Protein 335 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAGTTGTCATGCATCATTCTGGGGCAG |
| Internal bar code: | GCTCGGGCATACACGTCAAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 920 |
| LEAP-Seq percent confirming: | 99.6706 |
| LEAP-Seq n confirming: | 3934 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACTGGCTGACTTGACCA |
| Suggested primer 2: | ACCCACCCACTCACTCACTC |