Insertion junction: LMJ.RY0402.124566_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.124566
Chromosome:chromosome 17
Location:1906342
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g710300 PHC31 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 31 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGTCGACCTGTGCGACAACGTGTCCGTG
Internal bar code:GTCGTCCAGGGATTTGTCACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:112
LEAP-Seq percent confirming:99.921
LEAP-Seq n confirming:1264
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GATGCGACTTTCCTCCTCTG
Suggested primer 2:TTCAGGGCGTCAACAATGTA