| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.124568 |
| Chromosome: | chromosome 7 |
| Location: | 3296557 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334450 | EFH4 | EF-hand domain protein; (1 of 1) IPR007044 - Cyclodeaminase/cyclohydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATACGCTGTGATAGCATGCTATGGCGGC |
| Internal bar code: | TGATCGGTACGAGGTGTTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 232 |
| LEAP-Seq percent confirming: | 98.6487 |
| LEAP-Seq n confirming: | 219 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGAGGGAGTTTTGGTGG |
| Suggested primer 2: | GTCTTCTCTGGCGTAGGCAC |