| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.124574 |
| Chromosome: | chromosome 4 |
| Location: | 1341791 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214600 | (1 of 30) 3.1.3.48 - Protein-tyrosine-phosphatase / PTPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCGCTCCCAAGCGCACCACCCCAGCCC |
| Internal bar code: | GTTAATCGATTAAGCCAACAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 504 |
| LEAP-Seq percent confirming: | 99.6732 |
| LEAP-Seq n confirming: | 305 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTAAAATCGCCCAAACCC |
| Suggested primer 2: | GTTACTGCTGTCCTCGAGCC |