| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124577 |
| Chromosome: | chromosome 7 |
| Location: | 575525 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g316600 | MKP5 | (1 of 3) K04459 - dual specificity MAP kinase phosphatase (DUSP, MKP); Dual-specificity protein phosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGCCGGAAGTTGAGGAGCGCGGAAAGA |
| Internal bar code: | ACGGCTTCGTCGTGAAGGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 208 |
| LEAP-Seq percent confirming: | 87.94 |
| LEAP-Seq n confirming: | 1349 |
| LEAP-Seq n nonconfirming: | 185 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAATCAGTTCACTCCAGC |
| Suggested primer 2: | CACTAGTCGTGTGCCTGGAA |