Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.124600 |
Chromosome: | chromosome_7 |
Location: | 5524563 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g351150 | FA2 | Flagellar Autonomy Protein, NIMA family kinase | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCACGCCGGCGCGCCCTTCGCGACGCACTA |
Internal bar code: | GGGGGGCTAACAACTAGCACAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 280 |
LEAP-Seq percent confirming: | 98.3308 |
LEAP-Seq n confirming: | 648 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCCACCTCACACACAACGA |
Suggested primer 2: | GTATCTTGCACAGGGGCATT |