| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.124600 |
| Chromosome: | chromosome 14 |
| Location: | 296884 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609750 | (1 of 6) PF03351 - DOMON domain (DOMON) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCATGCACCAACCTGCACCCGATGCACG |
| Internal bar code: | GACACTGGGGCCGGCGCCGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 921 |
| LEAP-Seq percent confirming: | 99.3296 |
| LEAP-Seq n confirming: | 17780 |
| LEAP-Seq n nonconfirming: | 120 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTATCTGGTCGGTCAGGC |
| Suggested primer 2: | GGGAGTCTGAGAACGAGCAC |