| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.124621 |
| Chromosome: | chromosome 8 |
| Location: | 6481 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCGAGAAACCCAGCCGAAACTCGGGCG |
| Internal bar code: | GAATAATATATACGAATTCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 796 |
| LEAP-Seq percent confirming: | 99.1501 |
| LEAP-Seq n confirming: | 350 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGAGGTGACAGACAGGGT |
| Suggested primer 2: | CGGCCACATAAACTGCCTAT |